Of magnitude reduced than the typical content determination in the corresponding intensities. When the residual will not be extremely really serious, it will not influence the sample determination. three.two.2. Limit of detection, limit of quantification and linearity The results on the regression evaluation for each marker component, along with the LOD and LOQ values, were shown in Table two. The calibration curves had been determined according to the relative content material of each compound in YQJZG. All calibration curves showed good linearity with correlation coefficients (R2) within the variety from 0.9990 to 0.9998. three.2.three. Stability, precision and accuracy The sample options have been steady inside 24 h at 4 (RSD two.86 ). These data indicated that this created methodTable two Calibration curves and limit of detection for eight marker elements in YQJZG. Analytes GRg1 GRe GRb1 TEO IN HPD AG CCM Calibration curve y y y y y y y y = = = = = = = = 35.5467 21.2182 58.6352 1027.01 1695.25 1392.21 3947.57 446.023 x41.0527 x83.5767 x130.663 x+ 956.703 x+ 5538.88 x+ 45380.two x9292.51 x+ 17976.six R2 0.9998 0.9996 0.9996 0.9993 0.9990 0.9990 0.9999 0.was dependable and stable for the quantification of marker elements in YQJZG (Table 3). Six independent mixed normal options had been ready and analyzed for evaluating the instrument precision (RSD two.91 ). The outcomes from the precision determination have been shown in Table 3. The RSD values for the repeatability and intermediate precision had been significantly less than 3.54 and 2.88 respectively. According to the outcomes, this quantitative strategy has great precision and has access to satisfy the specifications with the experiment. As shown in Table 3, the average recovery rates and RSD values of every target element had been as much as the standards of the Chinese Pharmacopoeia (2020 edition). This analytical method was manifested to be trusted. three.3. Determination of samples The proposed UPLC-MS/MS strategy was applied to analyze the eight compounds in three batches of YQJZG. Multi-channel MRM chromatograms of sample solutions have been recorded (Fig. five). The contents of marker elements in YQJZG have been calculated employing the regression equations for the peak area versus concentrations. Table four showed the contents of marker components in YQJZG.Linear range (ng/mL) 13.0804.64 11.XTP3TPA, Human (His) 8818.PODXL Protein Biological Activity 80 14.PMID:24518703 7432.65 47.4714.72 35.1451.36 492.80928.00 15.9259.20 161.60454.LOD (ng/mL) 0.18 0.15 0.11 0.02 0.02 0.04 0.04 0.LOQ (ng/mL) 0.43 0.57 0.39 0.08 0.11 0.22 0.11 0.Note: Within the regression equation y = a x+ b, exactly where y refers for the peak location and x refers for the concentration with the analytes (ng/mL).S. Guo, S. Hu, L. Jiang et al. Table three Repeatability, precision, accuracy and stability of eight marker elements in YQJZG (n = 6). Analytes Repeatability RSD ( ) Instrument precision RSD ( ) Intermediate precision RSD ( ) Stability RSD ( )Chinese Herbal Medicines 14 (2022) 324Accuracy Typical recovery ( ) 95.69 94.49 91.08 94.45 96.89 93.91 92.51 103.89 RSD ( ) three.26 three.15 three.72 three.81 3.17 two.01 two.15 1.GRg1 GRe GRb1 TEO IN HPD AG CCM3.53 1.97 2.91 2.15 1.75 1.36 1.30 1.2.14 two.90 1.91 0.33 1.48 0.91 1.03 1.2.25 1.08 1.35 two.79 two.87 1.46 0.17 0.2.85 2.67 1.05 two.43 two.53 two.20 2.74 two.Fig. five. Multi-channel MRM chromatography for sample options. (a) GRb1 channel; (b) GRe channel; (c) GRg1 channel; (d) TEO channel; (e) IN Channel; (f) HPD channel; (g) AG channel; (h) CCM channel.Table 4 Determination results of three batches of samples. Analytes GRg1 GRe GRb1 TEO IN HPD AG CCM Batch 1 ( ) 0.0182 0.0125 0.0238 0.0344 0.