In comparison to handle values.Toxicol Appl Pharmacol. Author manuscript; obtainable in
In comparison with manage values.Toxicol Appl Pharmacol. Author manuscript; out there in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptToxicol Appl Pharmacol. Author manuscript; offered in PMC 2015 September 15.Figure 3. TCE inhibition IL-6 production is maintained more than timePeritoneal macrophages have been incubated with LPS following isolation from untreated control mice or from mice exposed to TCE (0.five mgml) for up to 40 weeks. Culture supernatants had been examined for cytokines (imply SD). Drastically distinctive (0.05) compared to manage values.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure four. TCE inhibition of Il6 expression is maintained over timeCytokine gene expression was examined in peritoneal macrophages incubated with or without LPS immediately after isolation from untreated handle mice or from mice exposed to TCE (0.5 mgml) for up to 40 weeks. The data represents the imply SD. Substantially unique (0.05) in comparison with handle values.Toxicol Appl Pharmacol. Author manuscript; obtainable in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author ManuscriptFigure five. TCE alters expression of hepatic genes more than timeA. Gene expression in individual liver tissue isolated from untreated manage mice or from mice exposed to TCE (0.five mgml) for as much as 40 weeks. The information represents the imply SD from six individual micetreatmenttime point. Substantially various (0.05) compared to handle values. B. Relative protein levels (percentage reference protein GAPDH) of IL-6R in person livers from untreated handle mice or mice exposed to TCE (0.five mgml) for 16 weeks (imply SD).NIH-PA Author Manuscript NIH-PA Author ManuscriptToxicol Appl Pharmacol. Author manuscript; out there in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 6. TCE liver pathology correlates with loss of hepatic Il-6r expressionA. Liver pathology determined by immune cell infiltration and inflammation was assessed in mice exposed to TCE (0.5mgml) for 28, 34 or40 weeks. B. Equal amounts of liver protein from an untreated mouse have been separated in four lanes of SDS-PAGE, each and every of which were immunoblotted with pooled sera obtained from control MRL mice or mice treated with 0.5 mgml TCE for four or 40 weeks. C. Hepatic gene expression in from mice exposed to TCE (0.5 mgml) for 40 weeks was plotted against liver histopathology inside the identical mice. Gene expression LTC4 review values are shown in log scale as a result of ideal skewness. Regression p-values have been computed working with an F test on the null hypothesis of horizontal slope.Toxicol Appl Pharmacol. Author manuscript; out there in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 7. Submodel for parameter estimationA. An IL-6 submodel was created for estimating dose-dependent reduction within the fraction of IL-6 expressed by the macrophage. Points and error bars CCR2 MedChemExpress represent data and uncertainty, whilst solid and dashed lines are the mean and 95 self-assurance intervals from model predictions. B. Time-course pathology scores have been utilized to extrapolate liver pathology according to time of TCE exposure. Points and error bars represent data and uncertainty, though strong and dashed lines are the mean and 95 confidence intervals from model predictions.NIH-PA Author ManuscriptToxicol Appl Pharmacol. Author manuscript; accessible in PMC 2015 September 15.Gilbert et al.PageNIH.