Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample high-quality testing enables detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: General, the study gives a tool for non-invasive monitoring on the functional status of the endometrium, supporting biomedical niches for instance assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Region Tuscany (identification on the project) and Exosomics R D ProgrammePF09.Unveiling autologous blood doping: comparative analysis of various purification approaches for urinary extracellular vesicles pioneering miRNA biomarker investigation Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM College of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany, M chen, Germany; cAnimal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive different isolation strategies were evaluated: ultracentrifugation, membrane affinity, spin column chromatography, immunoaffinity and precipitation. Following EV characterization by nanoparticle tracking evaluation, western blotting, and transmission electron microscopy, total RNA was isolated and also a library for compact RNA sequencing was ready. The resultant successful approach was then applied to all the collected samples which were equally analysed regarding their EV distribution and miRNA content. Outcomes: The comparative analysis disclosed enormous discrepancies with respect to EV yield, population, and purity, also as RNA yield and detected miRNAs. By applying the very best performing strategy, which was according to immunoaffinity, significantly higher amounts of urinary EVs and numerous substantially differentially regulated miRNAs were observed following ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold certainly promising attempts for the clear separation of ABD and non-doped athletes. In addition, the included complicated comparative methodological evaluation contributes enormously to future standardization and comparability of urinary EV research. Funding: The existing project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic CD66c/CEACAM6 Proteins Recombinant Proteins Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the oxygen capacity by means of re-infusion of a person’s autologous red blood cells. It really is Parathyroid Hormone Receptor Proteins web consequently utilized by endurance athletes with a high degree of unreported situations, particularly due to the fact reputable procedures for unequivocal detection are nevertheless lacking. To su.