Atives [513]. Identification of compounds was obtained matching precise mass Exendin-4 References measurements, fragmentation pathways, and literature [482], confirmation of a number of the compound was also obtained by co-injection of reference typical. Contemplating phenolic constituents, the extraction with methanol for the plant element outcome much more favorable. This result indicate a distinct extraction behavior with the flavonoid glycosides and procyanidin derivatives in water and in methanol, this outcome is usually explained by the unique solubility from the compounds within the two solvents, by the presence of other constituents that will interfere with the extraction and which might be not measured, for instance sugars, olygosaccharides, as well as lipid and waxes, too as resulting from the different potential on the water and methanol to penetrate dried plant material.Table two. Quantitative phytochemical determinations of P. kotschyi Nocodazole In Vivo Leaves and stem bark extracts . pValue Total phenolic content (mg GAE/g) Total flavonoid content (mg RE/g) Total phenolic acids content material (mg CAE/g) Total flavanols content (mg CE/g) 0.0001 0.0001 0.0001 0.0001 Leaves Methanol 127.18 1.85 b 43.79 0.30 b 24.88 0.58 c 7.77 0.06 b Leaves Water 126.81 0.89 b 44.95 0.38 a 63.58 2.00 a five.99 0.05 d Stem Bark Methanol 131.83 1.81 a 0.63 0.17 d 54.44 2.91 b 14.14 0.11 a Stem Bark Water 125.67 0.89 b three.30 0.14 c 53.76 4.01 b six.24 0.01 c Values are reported as mean S.D. GAE: Gallic acid equivalent; RE: Rutin equivalent; CAE: Caffeic acid equivalent; CE: Catechin equivalent. a line smart values with similar superscripts of this variety indicate no significant distinction among extracts (p 0.05).Table three. Identified and quantified constituents of P. kotschyi leaves and stem bark extracts using LC-DAD-MSn (mg/g), indicate confirmed identification by reference compound comparison. Number indicating compounds are the 1 applied for the structure of the main compounds reported in Chart 1 and of LC-MS chromatograms of Chart 2.Retention Time (min) 2.three Identification P1 prodelphynidin gallocatechingallocatechin P2 prodelphynidin B3 isomer 1 P3 prodelphynidin gallocatechingallocatechin P4 prodelphynidin B3 isomer two P5 prodelphynidin gallocatechingallocatechin P6 epigallocatechin P7 prodelphynidin B3 isomer 3 P8 prodelphinidin B3 isomer 4 P9 prodelphinidin B3 P10 procyanidin B2 P11 gallocatechin P12 procyanidin B dimer [M-H]- HR-MS Molecular Formula (Precise Mass) C30H25O14 (609.1244) C30H25O13 (593.1295) C30H25O14 (609.1244) C30H25O13 (593.1295) C30H25O14 (609.1244) C15H13O7 (305.0661) C30H25O13 (593.1295) C30H25O13 (593.1295) C30H25O13 (593.1295) C30H25O12 (577.1346) C15H13O7 (305.0661) C30H25O12 (577.1346) Fragments Leaves Methanol Leaves Water Stem Bark Methanol Stem Bark Water609.441 423 305 297 255 467 441 425 407 289 441 423 305 297 255 467 441 425 407 289 441 423 305 297 255 261 219 218 179 165 125 467 441 425 407 289 467 441 425 407 289 467 441 425 407 289 451 425 407 289 261 219 218 179 165 125 452 425 407n.d.n.d.0.74 0.0.15 0.2.593.1.55 0.0.36 0.0.94 0.0.27 0.3.609.n.d.n.d.0.47 0.0.08 0.five.593.n.d.n.d.1.06 0.0.16 0.5.609.n.d.n.d.0.97 0.0.09 0.6.six six.7 7.0 7.three 7.7 7.9 eight.305.0715 593.132 593.132 593.1332 577.11394 305.0742 577.three.32 0.02 1.63 0.01 n.d. n.d. two.08 0.01 n.d. three.57 0.0.71 0.01 0.77 0.02 0.77 0.01 0.77 0.02 1.22 0.02 0.71 0.01 1.45 0.1.69 0.02 9.20 0.02 1.35 0.02 1.32 0.02 3.46 0.02 1.34 0.01 three.28 0.0.63 0.01 0.60 0.01 0.31 0.01 0.57 0.01 1.26 0.02 0.45 0.01 1.62 0.Antioxidants 2021, ten,8 ofTable three. Cont.Retention Time.