Ted the hilar adipose tissue (inset, upper correct corner). This case also showed papillary functions focally (inset, reduced appropriate corner). SMARCB1 deficient medullary RCC, overlapping with collecting duct carcinoma (in-filtrative cords and tubules), with frequent angioinvasion, peritumoral neutrophils (D) and evidence on the characteristic sickled erythrocytes (inset, lower right corner, arrow). The tumor showed full loss of INI1 immunoexpression (in-ternal optimistic handle in adjacent lymphocytes and vessels). Tubulocystic renal cell carcinoma, becoming composed of tu-bulocystic structures filled by eosinophilic cells with prominent hobnailing and higher grade nuclei, inside a hypocellular fi-brotic stroma (E). A case of a collision tumor, with presence of a pRCC with classic morphology occurring inside the middle of an oncocytoma (F). CK7 highlights the pRCC (inset).Biomedicines 2021, 9,12 ofFigure 9. Eosinophilic vacuolated tumor from the kidney. The tumor is composed of cells Isoprothiolane Description arranged in smaller nests and cords, with eosinophilic cytoplasm and round 1-Phenylethan-1-One supplier nuclei with prominent nucleoli resembling oncocytoma, but the cytoplasm of tumor cells is remarkably vacuolated (modest and huge clear vacuoles) along the entire tumor (A). Succinate dehydrogenase deficient renal cell carcinoma. The tumor is classically composed of tubules and nests of mainly eosinophilic cells, with flocculent cytoplasm (B) and with vacuoles containing clear or slightly eosinophilic fluid, providing a bubbly look (C), but any morphology may well be observed, like rare papillary functions. The diagnosis is confirmed by the loss of expression of SDHB, with internal optimistic handle inside the adjacent renal tubules (inset, major proper). Notice that SDHA expression is retained (inset, bottom proper). Fumarate hydratase deficient renal cell carcinoma. The tumor showed a mixture of patterns, with strong, tubular, cystic and papillary regions (D). Many tumor cells presented the standard eosinophilic cytoplasm, round nuclei with prominent eosinophilic nucleoli surrounded by a clear halo (inset, top correct), and showed the loss of cytoplasmic granular expression of fumarate hydratase in tumor cells (retained in infiltrating lymphocytes and in stromal vessels, inset, bottom right).Some strong renal tumors with eosinophilic cytoplasm may also show places with papillary development. Such tumor sorts consist of succinate dehydrogenase (SDH) deficient RCC, eosinophilic solid and cystic RCC (ESC RCC) and eosinophilic vacuolated tumor (EVT). 4 situations of SDH deficient RCC had been documented (Figure 9). Three eosinophilic tumors with strong and cystic places had been classified as ESC RCC and 1 fulfilled the criteria of EVT. Amongst MiT family members translocation RCC, 11 were identified as TFE3 translocated RCC, 6 as TFEB translocated RCCs and a single TFEB-amplified RCC. Presence of TFEB amplification was confirmed by FISH (Figure 10). All TFEB-altered RCCs expressed melanocytic markers.Biomedicines 2021, 9,13 ofFigure ten. TFE3-translocated renal cell carcinoma. The tumor shows papillary architecture and clear cells (A) but can present with any morphology. Robust, diffuse positivity for TFE3 by immunohistochemistry strongly suggests the diagnosis (inset, proper upper corner), which was confirmed by break-apart FISH (inset, suitable decrease corner). TFEB-translocated renal cell carcinoma. Notice the admixture of clear cells and eosinophilic cells, also with all the presence of a second population of smaller sized cells in clusters, focally surrounding or di.