Kb) Abbreviations ANOVA: evaluation of variance; APP: amyloid precursor protein; BSE: bovine spongiform encephalopathy; Bv109I: bank vole genetic line carrying isoleucine homozygosity at PRNP codon 109; Bv109M: bank vole genetic line carrying methionine homozygosity at PRNP codon 109; CNS: central nervous technique; CSF: cerebrospinal fluid; CTF: C-terminal fragment; dCJD: CJD triggered by dura mater graft; DW: diffusion-weighted; EEG: electroencephalogram; ELISA: enzyme-linked immunosorbent assay; FLAIR: fluid attenuated inversion recovery; GFAP: glial fibrillary acidic protein; HLA: human leukocyte antigen; LFB: luxol speedy blue; M: methionine; mAb: monoclonal antibody; MRI: magnetic resonance imaging; np-CJDMM1: CJDMM1 not associated with PrPSc plaque-like deposits in white matter; PAS: periodic acid-Schiff; PBS: phosphate buffered saline; p-CJDMM1: CJDMM1 related with PrPSc plaque-like deposits in white matter; PK: proteinase K; PLP: myelin proteolipid protein; PMSF: phenylmethylsulfonyl fluoride; PRNP: prion protein gene; PrPC: cellular prion protein; PrPSc: Scrapie prion protein; sCJD: sporadic Creutzfeldt-Jakob illness; TBS: Tris buffered saline; TH: total homogenate; TSA: thermo-solubilization assay; t-tau: total tau; V: valine; vCJD: variant CJD Acknowledgements We want to thank Barbara Polischi M.Sc., Silvia Piras, B.Sc., Stefano Marcon and Geraldina Riccardi for their important technical assistance; Claudia D’Agostino, DVM, Paolo Frassanito, Shimon Simson and the technical employees of your animal facility at ISS for their superb animal care and perform. Funding This work was supported by RFO grants in the University of Bologna along with the Gino Galletti Foundation (to PP) and by the grant RF-2009-1,474,624 from the Italian Ministry of Well being (to RN). AR is funded by the Netherlands Ministry of Well being (RIVM). Availability of data and supplies The datasets utilized and analyzed during the current study are out there from the corresponding author on reasonable request. Authors’ contributions All authors produced substantial contributions to conception and style, and/or acquisition of information, and/or analysis and interpretation of information. The first draft was written by PP, MR and DS and finalized by PP. All authors gave final approval of your version to become submitted. Study conception and style: PP, MR and DS. Acquisition, analysis, and interpretation of information: MR, DS, MDB, SB, AWL, LP, SC, AR, RN, PP. Writing from the manuscript PP, MR and DS. Vital revision with the manuscript: all authors. Ethics approval and consent to participate Information collection of clinically suspected instances is definitely an integral part of the National CJD surveillance study, which was approved by the Ethic Committee on the Istituto Superiore di Sanit(CE-ISS 09/266 on 29 may perhaps 2009). Bank voles have been obtained in the breeding colony at the Istituto Superiore di Sanit(ISS), Italy. IL-13 Protein Human Experiments involving animals adhered towards the guidelines contained in the Italian Legislative Decree 116/92, which transposed the European Directive 86/609/EEC on Laboratory Animal Protection, then in the Legislative Decree 26/2014, which transposed the European Directive 2010/63/UE on Laboratory Animal Protection. The analysis protocol was performed below the supervision with the Service for Biotechnology and Animal Welfare with the ISS. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests.Rossi et al. Acta Neuropathologica Communications (2017) five:Page 12.