To check the binding of Msf fragments to this location of Vn, a biotinylated peptide spanning human Vn residues 438 (VA-26: VTRGDVF TMPEDEYTVYDDGEEKNNA) and a handle peptide ended up utilized to ELISA plates coated with recombinant Msf fragments

ments were performed working with an endogenous ERC marker, Rab11 in addition to a transfected marker, Rab11-FIP2. We found that SUMOylation includes a dominant negative impact on tubular localization of…