Erapeutics. We are pleased to offer 2,2′-dipicolylamine phosphoramidite. This product was manufactured and developed by Syntrix Biosystems Inc. Patents Pending. For Research Use Only. orderinG inForMation
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TC aND TCO: NEw TRiCyCLiC fLUOREsCENT CyTiDiNE aNaLOgUEs wiTh a VERy BRighT fUTURE
Glen Research has offered fluorescent nucleosides for a long time: 2-Aminopurine deoxynucleoside and Etheno-dA have been in our catalog for more than 15 years. More recently, we have become interested in the potential for dC analogues to offer interesting fluorescence properties while maintaining excellent base pairing behavior with dG. In partnership with Berry and Associates, we introduced pyrrolodC1, which has intriguing properties that allow its exact position in a duplex to be determined. And, recently, we have pointed out the fluorescence properties of AP-dC (G-Clamp).2 Now we are happy to introduce the tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo. Tricyclic dC base analogues have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure.3-5 This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. 1,3-diaza-2-oxoPhenothiazine (tc) Wilhelmsson and coworkers have demonstrated that tC is unique in the group of fluorescent base analogues that mimic cytosine.5 As might be predicted, the structure of duplex DNA containing tC retains the native B-form. Melting studies on duplexes containing tC have demonstrated that it base pairs strongly with G and the melting temperatures of duplexes are increased by 2.7over the equivalent C-containing duplex. tC is strongly fluorescent with an absorption maximum at 385nm, which is far outside the wavelength of DNA absoption. Its emission maximum is at 500nm.4 Of great significance is the fact that the fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA.188125-42-0 InChIKey The average quantum yield was 0.134448-10-5 Molecular Weight 21 for single stranded DNA and 0.PMID:30855926 19 for duplex DNA. Also, the fluorescence characteristics of tC were not sensitive to neighboring base combinations.4 This combination of characteristics make tC one of the most interesting fluorescent bases so far evaluated. 6
1,3-diaza-2-oxoPhenoxazine (tco) Like its sulfur-containing cousin, tCo is also a very bright fluorophore. It has been reported to be 25-50 times brighter than 2-aminopurine.3 The reason for this is twofold. First, tCo has a large extinction coefficient with an E360 of 9,000 L/mol.cm. Second, unlike other nucleoside analogs, tCo is not severely quenched when in doublestranded DNA. Peter Sandin and colleagues did an exhaustive study of neighboring base effects in single- and double-stranded oligos, determining the quantum yield of fluorescence (QY) for all permutations of bases immediately 5′ and 3′ to tC o when the oligonucleotide is single versus double-stranded.3 They found that on average, the QY is 0.30 for single-stranded oligos and a quite respectable 0.21 when double-stranded, making tCo the brightest fluorescent nucleoside analogue in doublestranded DNA reported so far.
sPectral characteristics Figure 2 shows the absorption and emission spectra of the oligo 5′-ATC GXT CAT GAT G-3′, where X is tC, as determined in house. The absorption maximum was found to be at 392nm with no shift in wavelength in duplex DNA. The emission maximum was found to be.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com