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KDEL monoclonal antibody (10C3)
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The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus. Western Blot analysis of ENZ-ABS679: Lane 1: MW Marker, Lane 2: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 3: Mouse Liver Microsomes , Lane 4: Rat Liver Microsomes (ADI-LYT-RM100), Lane 5: HeLa (heat shocked), (cell lysate) . Western Blot analysis of ENZ-ABS679: Lane 1: MW Marker, Lane 2: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 3: Mouse Liver Microsomes , Lane 4: Rat Liver Microsomes (ADI-LYT-RM100), Lane 5: HeLa (heat shocked), (cell lysate) .
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| Alternative Name Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine | Application ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB | Application Notes Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.{{548470-11-7} medchemexpress|{548470-11-7} Technical Information|{548470-11-7} References|{548470-11-7} manufacturer} | Clone 10C3 | Formulation Liquid.{{374683-24-6} medchemexpress|{374683-24-6} Biological Activity|{374683-24-6} References|{374683-24-6} manufacturer} In PBS, pH 7.PMID:31424719 2, containing 50% glycerol and 0.09% sodium azide. | GenBank ID M14050 | Host Mouse | Immunogen Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78. | Isotype IgG2a | Recommendation Dilutions/Conditions Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Source Purified from hybridoma tissue culture supernatant. | Species Reactivity Avian, Insect, Mammalian, Plant, Yeast | Specificity Recognizes proteins containing the KDEL sequence. | UniProt ID P06761 | Unit of Measure (UM) µg