Hinese Academy of Healthcare Sciences and Peking Union Health-related College, Chengdu, Sichuan, 610052, China, Chengdu, China (People`s Republic)Introduction: IFN-induced exosomes (Exo-IFN) may well effect on viral dissemination or antiviral immunity and as a result involve in the pathogenesis of lots of infectious pathogens. Having said that, small is identified about its underlying mechanisms. To better comprehend how Exo-IFN performs its anti-viral impact, we employed RNA sequencing evaluation to B7-DC/PD-L2 Proteins custom synthesis discover the exosomal expression profiles of lncRNA and mRNA associated to viral infections. We hypothesized that exosomes can regulate viral infection via transmitting enclosedspecific lncRNAs into neighbouring cells to inhibit viral replication. Approaches: Exosomes were purified from A549 with/ without having IFN therapy by serial centrifugation followed by sucrose density gradient purification, and CD54/ICAM-1 Proteins Recombinant Proteins characterized by TEM and Western Blot. ELISA assay have been performed on purified exosome fractions to demonstrate that they are free of charge of IFN. ZIKV replication was assayed by real-time PCR. Final results: ZIKV replication was substantially suppressed in A549 cells pre-treated with Exo-IFN followed by ZIKV infection. In addition, we discovered that anti-ZIKV impact of Exo-IFN is IFN-independent since ZIKV replication was also decreased in U5A cells (IFN-/ receptor IFNAR deficient) pre-treated with Exo-IFN . Similar benefits had been observed in Dengue virus and HCV infections. RNA sequencing analysis located many lncRNAs and mRNAs had been differentially expressed and function annotation and pathway evaluation demonstrated that the differentially expressed genes have been involved in a lot of functions and pathways, which includes anti-viral infection. To validate the RNA sequencing analysis outcomes, some lncRNAs have been selected to test their expression levels by qPCR. We are inside the process of deciphering the mechanism employed by these exosomal lncRNAs in anti-viral activty independent of inteferon. Summary/conclusion: We think that understanding the anti-viral functional molecules wrapped in exosomes might support design and style exosomes as effective autos for antiviral therapy. Funding: Chinese Academy of Healthcare Sciences Innovation Fund for Health-related Sciences (2016-12M325)JOURNAL OF EXTRACELLULAR VESICLESPF06: Advances in EV Quantification and Characterization Chairs: Estefan Lozano-Andr ; Kenneth Witwer Place: Level 3, Hall A 15:306:PF06.Exosome quantification by ELISA and Flowcytometry applying anti-CD9 antibody Naoki Hataa, Hiroyuki Kogurea, Hikaru Sonodab and Chihiro Okadabathe exosomes and control samples were shown by CellStream flow cytometer. The robust sensitivity of ELISA and CellStream flow cytometer with use of the validated CD9 antibody would offer an informative platform for measuring exosomes. Funding: No fundings.Luminex corporation, Tokyo, Japan; bHakarel Inc, Osaka, JapanIntroduction: Quantifying and characterizing exosomes inside a reproducible and reputable manner has been challenging due to their little sizes, of which the ranges are from 30 to 150 nm in diameter. The evaluation employed to become mainly performed with either the electric microscopy or the nanoparticle tracking evaluation; having said that, these strategies are low throughput and not sufficient for the quantification particularly inside the substantial and heterogeneous populations. Also, attempts to analyse exosomes applying regular PMT-based flow cytometers has been hampered by the limit of detection of such compact particles and low refractive index. Right here, to overcome these limitatio.